![]() ![]() if you have worked with plastic parts or you have automotive experience we consider these as an advantage.you are precise, able to make decisions on your own,. ![]()
0 Comments
![]() ![]() If you have any questions or need an extra picture or two, feel free to reach out. ⋆ Due to sanitary reasons, we do not accept returns. ⋆ We pack everything as carefully as we can and use recycled boxes & packing materials as often as we are able. ⋆ Each item we list is meticulously cleaned with the appropriate method relevant to each vintage piece. ⋆ We try to present colors as accurately as we can, but keep in mind that colors may show up differently on different screens and devices. For this reason, we include written measurements in our descriptions. ⋆ Pictures might not be the best judge of size when it comes to some items. ⋆ Please take your time to review the photos closely as they are part of the item's description we do our best to ensure that the pictures show all aspects of the items, good and not so good, so that any noticeable flaws are visible. ⋆ All of our vintage items are sold as is. With a unique two-sided serrated blade with fork tipĬanadian Buyers - All list prices include applicable GST/HST I usually insist on doing all of my own cooking and baking, but this time, Dalo's will be doing the cake.Vintage Dalo - Universal Double-Cut - Two-Sided Serrated Knife with All Purpose Fork Tip and wooden handle I'm not even a cake person, but it was wonderful! I am having a big cookout in September and I will be ordering another cake. Everyone raved about how delicious it was. When I picked the cake up the morning of my wedding day, I was so pleased when I saw the beautiful job he did. I gave a couple of pictures to Paul and explained what I wanted. User ( 02:32) I ordered a wedding cake a bit close to my wedding date. I'm more partial to their pastries, but there weren't many birthday parties growing up that didn't have their pizza. Everyone was raving about the cake! User ( 18:35) Dalo's makes my favorite pizza and sweets! Their cookies are always well made and nicely decorated, the donuts and brownies are the best in Berwick, my favorite is their peanut butter brownie! And their pizza is always yummy, it's best eaten in the summer! User ( 09:55) Great local bakery/pizzeria. Ordered our wedding cake, from Georgia, and not only was it delicious but it was beautiful beyond our expectation. User ( 01:46) Paul at Dalo's was fantastic to work with. Breads! Plus pizzas to go in their cooler grab throw on grill! User ( 20:14) Great pizza and baked goods. Bakery pizza and toppings yes can accommodate parties. The chocolate peanut butter brownies plus their signature Peanut Butter bismarks. The glazed cream filled donuts better get there early. Had some issues with a cracked cake and was well taken care of. User ( 18:15) Always delicious and fresh love their pizza and cakes. Overall, it has it's good and bad, but I think it comes down to the person. I am not a big fan of the cannoli nor the pizza(seriously, that isn't pizza, sorry). I always order some seeded Kaiser rolls for my dad when I go down to Florida to visit him. Also custom orders to your liking User ( 06:30) Love their baked goods, pizza is great as well, great price for the size! User ( 10:12) Great pizza and awesome dessert. Restaurants near Dalos Bakery 1201 Freas Ave, Berwick, PA 18603-1611 Tuzzi Baking Co 7 of 27 Restaurants in Berwick 35 reviews 506 Washington St 0. User ( 01:08) Great pizza and full bakery. Also the cinnamon buns and other donuts/pastries are also amazing. User ( 21:27) Huge fan of the Pizza and the cookies are great too! Many people that don't like the pizza don't realize that its provolone rather than mozzarella so it melts and tastes different than normal mozzarella pizza(personally its my favorite pizza in the Berwick-Bloomsburg area) also it is Sicilian deep dish too, and for the price and extra toppings if you want any it is super affordable. What Others Say about this business: User ( 05:42) Always great service and fabulous foods. ![]() ![]() To be sure, younger workers have always been more inclined to switch jobs. “While their parents may have demonstrated admirable work ethic and loyalty, they often didn’t have much to show for it in terms of personal fulfillment and financial stability.” The 26-year-old, who has switched jobs four times since graduating university in 2017, told Fortune that the younger generation is jaded by what they’ve seen. Leigh, a data scientist and personal finance content creator, who requested anonymity for safety purposes, said that Gen Zers have grown up observing how being employed in the same job for years impacted their parents. So millennials responded in kind.ĭata bears this out research firm Gallup found that in 2016, 21% of millennials said they wanted to switch jobs, three times higher than non-millennials at the time. The era of mass layoffs truly began in the 1970s but the battle scars of the Great Recession in 2008 were kindling on a bonfire. By the time millennials entered the workforce in the 21st century-around the same time as another late ‘90s policy revolution took place, China’s entrance into the World Trade Organization-the middle class was in the grips of decline and companies were rapidly losing loyalty to workers. ![]() Ennui about work surfaced in pop culture, memorably in the Gen X workplace classic Office Space. In retrospect, perhaps a slight change was notable in the Gen X era of the 1990s, when major shifts got under way, particularly the signing of NAFTA, which led to massive job losses, especially in manufacturing. Although countless changes hit the economy and the workforce in ensuing decades-from the “ Great Inflation” of the 1970s through the “ Masters of the Universe” 1980s turbocharged by finance-the idea of staying loyal to one’s employer never quite wavered. Organization men appeared throughout pop culture, for instance memorably in Billy Wilder's 1960 classic, The Apartment. It covered the midcentury mentality of loyalty to one’s company for one’s entire career, especially at the managerial level. Millennials were the first to earn the title of “ job-hoppers”-referring to workers who are not held back by employer loyalty and prefer to move around more often than their older counterparts.Īt one point, changing jobs regularly was an unusual concept, as reported by Fortune all the way back in 1953 with “ The Transients,” a feature story that became a best-selling book called “ The Organization Man” by former editor William H. Abbatiello says the new findings show a generational shift is underway. Even the professor who coined the term predicted it would end this year. After surging in 2021 quit rates fell last month back down to pre-pandemic levels of 2.4% across industries, pointing to an end of the so-called Great Resignation. When the Great Resignation first kicked off, Zippia, a recruitment and job insights platform, found that a bulk of those who quit were in the 18 to 29 age bracket, which includes a mix of old Gen Zers and young millennials. “I think what we saw at the beginning of the Great Resignation, I would say we saw more by industry or role.” Quitting was more about the type of work being done instead of a generational attitude, he said. ![]() The common reason cited by 44% of the workers considering quitting their jobs is overwork.Īnthony Abbatiello, partner and workforce transformation practice lead at PwC, told Fortune that there’s a shift under way. Gen Z workers, aged between 18 and 26, make up about 35% of those wanting to quit, while millennials (aged 27 to 42) are another 31%. But Gen Z has other ideas, dreams even.Ī survey by PwC, one of the “Big Four” consulting firms that periodically weighs in on all things workplace and future of work-related, found that a quarter of employees globally want to shift jobs in the next 12 months. It seems to be losing steam in 2023-if it’s not dead entirely-as an era of mass layoffs and “sticky” inflation displaces the overheated economy of the pandemic recovery. What began that year stretched well into 2022, with 50.5 million people quitting their jobs, and took on cultural resonance-Beyoncé even wrote a song crowning the trend as a pop culture moment and countless viral TikTok (or #QuitTok) videos were made about it. alone, a record 47.8 million people quit their jobs in search of greener pastures. ![]() ![]() We are now two years past the Great Resignation, when tens of millions of workers suddenly left for new opportunities, either because of burnout, the desire for better working conditions or some combination of the two. ![]() ![]() ![]() 20/120 (baseball) - S etting to easily time :20 between pitches and :120 between innings. The 20 second timer gives an initial vibration once engaged, then again at :10 remaining, then a long vibration when the timer reaches 0.25/60 (football) - 60 second timer has a vibration warning 15 seconds remaining, then again when the timer reaches 0.The 25 and 40 second play clock has a quick vibration warning with 25 seconds remaining, then with 10 seconds remaining, then will vibrate in 1 second increments for the last 5 seconds. 25/40 (football) - Warning vibration with :25 remaining on the :40 play clock.Audible sound occurs when power on if batteries need replacing.Automatic 30 minute shut off to conserve battery life.Simple flip the toggle switch up to start the longer timer or down for the shorter.Unlike a wrist watch, there are no tiny buttons.Tempered steel black powder coat belt clip is secure to stay in place during the most grueling conditions.Each timer mode comes with a sport-specific vibration warning.Compatible for football, lacrosse and baseball with 4 time settings.LACROSSE: Time defensive zone clearance and timeouts. Meets NEW NFHS Football timing rules started in 2019. Meets NCAA 2023 Action Clock Update.įOOTBALL: Time the 25/40 or 25/60 second play clock used in High School and NCAA. If you have already purchased a RefSmart timer on or before and would like it reprogrammed for the 2023 NCAA Baseball Rule changes please visit the link below.īe on top of your game with this Universal Game Day Timer by RefSmart - for baseball, football and lacrosse.īASEBALL: Time the 20/120 second clocks between pitches and innings. Now includes warning vibration at 10 seconds remaining of the 20 second action clock. Updated for the 2023 NCAA Baseball Season. Tennessee Collegiate Umpire Association.Santa Clara Valley Federation of Umpires.New York State Baseball Umpire Association.New Jersey State Interscholastic Athletic Association.New Hampshire Softball Umpires Association.Mississippi High School Activities Association.Louisiana High School Officials Association. ![]() Kentucky High School Athletic Association.Kansas State High School Athletic Association.Indiana High School Athletic Association.Arizona Community College Athletic Conference.Alabama High School Athletic Association.If you flip too fast sometimes it does not reset. Good timer, I practiced at home before using in a game. The 120 second timer also helps keep track of length of timeouts during a game. It' s easy to use & works perfectly for lacrosse. I bought this timer a few weeks ago for the lacrosse games I officiate, since I was elevated to some of the higher youth teams that keep counts. Great little timer.works every time.light weight.no problems Posted by Robert R Kellogg on 17th Jun 2021 It is lightweight yet durable.ĭecent functionality, weak on construction, stopped working after the first year. This is a high quality product which makes the back judges job much easier. Second warning vibration with 15 seconds remainingĮasy to use, set to go out of the box, functionality of the instrument works perfectly.First warning vibration with 30 seconds remaining.Toggle up for 120 seconds between innings.Provides silent warning vibrations to eliminate distractions.Unlike a wrist watch there are no tiny buttons Easy to use in all weather conditions.Easily time the NCAA Action Pitch Clock.Low battery warning: When you turn the timer on, it will beep if the battery is running low.īaseball Update:Timers will be updated for the 2023 NCAA baseball rules.New! Baseball 20/90 function now included.Can be programmed to be audible for 20/120 Lacrosse.The 60 second timer has a vibration warning 15 seconds remaining, then again when the timer reaches 0.The 25 and 40 second play clock has a quick vibration warning with 10 seconds remaining, then will vibrate in 1 second increments for the last 5 seconds.Unlike a wrist watch there are no tiny buttons, but s imple click toggle switch.T empered steel black powder coat belt clip is secure to stay in place during the most grueling conditions.This one timer works for football (25/60 & 25/40), Baseball and Lacrosse.Easily time the play clock and time outs.You can change the timing options as you wish (instructions will be included).NFHS Football 25/60 (Vibrate Only), NCAA & Texas Football 25/40 (Vibrate Only), Lacrosse 20/120 (with Audible Tone) and NCAA Baseball 20/90 (Vibrate Only) - all programming options are included in this 1 Universal Timer. ![]() ![]() Here, you can check the firmware version and status of all devices and check if there is an update available or a schedule set to upgrade. In order to access a full overview of all devices on a site and their firmware status, please navigate to: Site-Wide > Configuration > Firmware management Contact Zyxel customer support if you want to unlock the firmware in order to upgrade to a later one. This shows Dedicated when the installed firmware is locked and Zyxel support has been installing the firmware for testing purposes. There are 4 firmware statuses that you may see when you're looking at the firmware status on the Status product page:ġ) General Availability - You have an older version and it's strongly recommended that you upgrade to the stable firmware version (or latest)Ģ) Stable - You have a stable firmware version and can upgrade to the latest version if you'd likeģ) Latest - You have the latest firmware version available already installedĤ) Dedicated - There is also a fourth firmware status called Dedicated. ** Firmware available ( but sometimes not required) Updating the firmware of your Nebula device is just a few clicks away! This tutorial should give you a quick overview of the possibilities of upgrading your devices.ġ) How do I know if I need to upgrade the firmware? Only the Anker Nebula Astro Portable offers you the option to hook your projector up with the web.This article shows how to firmware upgrade your Nebula device, how you know when to upgrade your firmware, what general availability, stable and latest mean. Most of the time, we want to connect our media devices to the Internet for practical reasons, such as watching the latest cat videos! The mini projector scene is catching up to this and some models are already equipped with WiFi functions. It's notable that battery times can vary depending on what you use your projector for, and can be less than the producer officially claims. The battery of the Nebula Astro Portable lasts for up 150,0 minutes, which is shorter than the Nebula Prizm 2's 2.421,0 minutes. This is a major advantage over the Anker Nebula Prizm 2, which requires a power outlet close to you. ![]() Only the Anker Nebula Astro Portable has batteries. Whether you're out with friends or hold a presentation at work: Batteries can't hurt in case you don't have access to a power outlet. ![]() The Anker Nebula Prizm 2 on the other hand has Full-HD, at a resolution of 1920 x 1080.Īs far as batteries go, we believe that it's very useful for your portable mini projector to have them. ![]() The Anker Nebula Astro Portable has a non-standard resolution of 854 x 480. Some mini projectors support HD, others Full HD, and then there's even True UHD or 4K mini projectors. the Anker Nebula Prizm 2's maximum performance are 200 Lumen.Īnother key consideration when purchasing a mini projector, is its resolution. The Anker Nebula Astro Portable can run at a maximum of 100 Lumen, which is less than the Anker Nebula Prizm 2 runs on. Let's have a closer look at some of them. Still, the two models have a couple of notable differences. Both mini projectors were produced by Anker. ![]() ![]() ![]() ![]() Strict user limits can make scaling difficult.įor more information, read the full QuickBooks review.įreshBooks: Best for high clientele countįreshBooks offers software solutions to freelancers, solopreneurs, small businesses and organizations that rely on contractors.Robust platform options may be more than simpler use cases need.Many key features are part of add-ons that drive up costs.Widely used and integrated, making it easy to implement and troubleshoot.Extensive tax support through add-ons and support from human experts. ![]() An all-in-one solution, especially for businesses that need to track physical resources.Easy handling of payroll, taxes, business bank accounts and more.Invoices and estimates, measured financial performance over time and generated reports and forecasts.Fully managed cashflow from accounts receivable to accounts payable.However, you can’t do both - it’s either the discount or the trial. You can get QuickBooks for 50% off for the first three months to save a little money upfront, or you can take advantage of the 30-day free trial to see if it’s the right fit for you. In some cases, like payroll, these features are part of distinct add-on subscriptions that increase the monthly base cost when included. The Simple Start and Essentials plans primarily include core accounting functions, with most advanced features like inventory and profitability projections available at only the higher tiers. The core QuickBooks product has four pricing tiers: Designed to help organizations of any size track income and expenses, inventory, production costs and a great deal more, QuickBooks is among the most widely used solutions in the business landscape today. The foremost name in business financial software, QuickBooks by Intuit is an industry powerhouse that has been around since the rise of digital systems. QuickBooks: Best for full-stack business resource management We’ve highlighted four major areas of functionality and identified which solutions fulfill these areas. But there is more to accounting than just managing a spreadsheet, and different industries have different financial concerns. No business lasts long without an accurate financial record and an effective system for managing cashflow. Top Xero competitors and alternatives: Comparison table Top Xero competitors and alternatives: Comparison table.In this article, we’ll take a closer look at several alternatives to Xero so you can choose the best software for your business. The tool boasts over 3.5 million users, but that doesn’t mean it’s a perfect fit for every use case. Featuring double-entry accounting, it’s designed to maximize ledger accuracy, error prevention and fraud protection. Xero is an accounting software solution aimed at small and mid-sized businesses, as well as professional accountants and bookkeepers. ![]() ![]() ![]() Kimberley Garner cuts a stylish figure in a flirty blue mini skirt as she enjoys an al fresco lunch with a male pal in west London ![]() Noah Cyrus steals the show (and all the space!) as she rocks a VERY wide-brimmed hat while viewing Yohji Yamamoto's PFW collection ![]() Sheldon Harnick dies at 99: Fiddler On The Roof lyricist was behind the hit song If I Were A Rich Man and had a rich history with Broadway musicals ![]() Katie Holmes shows off her bold and unique style in geometric sweater and jeans while stepping out in NYC Oti Mabuse cuts an glamorous figure in a bold floral gown while Charlotte Hawkins looks elegant as they lead the stars on day four of Royal Ascotĭemi Moore, 60, cuts a chic figure in a cream tweed suit she joins leggy Winnie Harlow and stylish Will Poulter at the Dior show during PFWīeautiful in blue! Crown Princess Victoria of Sweden is stunning in a shirt dress as she steps out for statue unveiling in StockholmĬristiano Ronaldo's girlfriend Georgina Rodríguez sizzles in a TINY thong bikini as she cuddles up to her beau during sun-soaked getawayĪlison Hammond delights fans with cameo in Stormzy's video - while Ruth Langsford matchmakes by declaring they make a 'lovely couple' The rapper is said to have gifted 5,000 tickets to faculty, staff and students from some Atlanta HBCUs including Clark Atlanta, Morehouse, Spelman, Morris Brown and the Interdenominational Theological Center, according to USA Today. 'He was respectful and gave her the heads up, and did not want to blindside her.'Īnd as evident in his lyrics he is reportedly trying very hard to 'keep the family unit,' despite him predominantly residing at his Wyoming ranch while Kim maintains ownership of their Hidden Hills mansion.Īs his album came to a close, West is said to have calmly walked off stage to cheers from the audience.Īlthough he is associated with being from Chicago, West was born in Atlanta, where there are currently no restrictions for large gatherings. 'Kanye did write a song about Kim and their marriage and Kim gave her input about it,' an insider told E! News. It does not store any personal data.That's all folks: Kanye stood on stage for 48 minutes before leavingīut despite the very personal track the YEEZY founder had given Kim a 'heads up,' and she reportedly gave her 'input,' on it. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The cookie is used to store the user consent for the cookies in the category "Performance". This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary". The cookie is used to store the user consent for the cookies in the category "Other. The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". The cookie is used to store the user consent for the cookies in the category "Analytics". These cookies ensure basic functionalities and security features of the website, anonymously. Necessary cookies are absolutely essential for the website to function properly. ![]() ![]() ![]() Aluminum Silver in color and typically bright-aluminum mesh is flexible, malleable and tends to be favored by the artistic user. $10.76.Sturdy and silver-gray in color, stainless steel mesh is requested when a unique, industrial or modern look is wanted. New 1pc Stainless Steel Flour Sieve Round Handheld Fine Mesh Sifter Baking Colan. Free shipping Stainless Steel Flour Sieve Kitchen Fine Mesh Oil Strainer Sifter Sugar Filter. AISI 304 is the most widely used conventional stainless steel and has excellent corrosion resistance in most environments.Kitchen Stainless Steel Flour Sieve Fine Mesh Oil Strainer Sifter Sugar Filter ! $2.92. Benefits of Expanded Metal Stainless Steel Mesh Stainless steel mesh is a metal product with a series of openings made of stainless steel wire or sheet. The Expanded Metal Company is a leader and innovator in the development and manufacture of expanded metal mesh products, combining more than a century of industrial experience with cutting edge technical capabilities and advanced production processes.We maintain stocks of stainless steel mesh, in a large range of mesh specifications. These include mild steel, galvanised steel, brass, phosphor bronze, copper, monel and aluminium. stocks woven wire mesh in stainless steel as well as a range of other metals. ![]() The materials used to make stainless steel mesh are: 201, 202, 304, 316, …Woven Wire Stainless Steel Mesh Robinson Wire Cloth Ltd. can handle all of your disc and extruder screen fabricating requirements.Stainless steel mesh is a metal product with a series of openings made of stainless steel wire or sheet. Aluminum mesh is lightweight, strong, has a high electrical conductivity, and a low melting point.Fabricated Wire Mesh Products Disc and Extruder Screens Fabricated Parts Basket Fabrication Disc and Extruder Screens As a supplier of industrial mesh for over 160 years, Edward J. Stainless Steel Woven …Galvanized steel Stainless wire mesh Aluminum Copper Brass Stainless wire mesh is especially useful because it is extremely chemical resistant, works with hot or cold liquids, and is easily cleaned. Great prices & free shipping on orders over $50 when you sign in or sign up for an account.25malx funny toys 304 Stainless Steel Woven Mesh 120 Mesh - Applied to air conditioning, purifier, range hood, air filter, dehumidifier and dust collector. ![]() Material Options For Expanded Stainless Steel Sheet Metal2pcs Stainless Steel Mesh Sieves Sifters Noodle Colander Deep Filter Spaghetti $16.24 Free shipping 2pcs/Set Stainless Steel Fine Wire Mesh Oil Stainer Flour Colander wood handle $12.99 Free shipping 2pcs Multi-function Noodle Strainer Convenient Mesh Strainer Stainless Pasta $13.10 Free shipping 2pcs Stainless Steel Mesh Sieves Sifters Noodle Colander Deep Filter Spaghetti $16.24 Free shipping 2pcs/Set Stainless Steel Fine Wire Mesh Oil Stainer Flour Colander wood handle $12.99 Free shipping 2pcs Multi-function Noodle Strainer Convenient Mesh Strainer Stainless Pasta $13.10 Free shipping Order Rsi N Americ WINDOW PROTECTION Use With EVOc Smartcap Rear Window Security Mesh Stainless Steel, SA100613 at. Additionally, it can keep the interior space ventilated and illuminated by natural light. Stainless steel mesh Stainless steel mesh is very durable and tough to ensure safety and security when it’s being used as a dividing screen to enclose a space. ![]() ![]() ![]() ![]() I've never taken a penny that doesn't belong to me, and the beauty of knowing the truth is you know the truth, and to me, it's just another battle that I have to face to fight for my freedom. "Honestly, what I can say, is these are genuinely ludicrous allegations. "It's ridiculous," Haart said at the time of the claims. While the first season focused primarily on Haart’s inspirational journey from a restrictive, ultra-Orthodox Jewish community to the fashion world, Season 2 finds her at a time of crisis. I look at that title, Brazen, and I realize it's who I want to be, I'm not there yet."Īt the time, there had been allegations made against the former La Perla creative director, calling her "phony" or "difficult to work for" and she faced legal allegations from Scaglia, who claimed in court docs that she "abused" her role as CEO of their shared company, Elite World Group, and used company funds for personal purchases before he fired her. You have a ways to go, you still think men know more than you, you still are seeking their approval,'" she said at the time. "I'm still a work in progress. "It's obviously not the way it was planned, but I learned in my life that every time I think to myself, 'OK Julia, you're strong, you've proven yourself,' I feel like something comes along and says, 'No, you're not done yet. She was simply a recently married 19-year-old girl when her mother chose to flee the community, so Batsheva not only started to question everything she’d learned but also felt abandoned, which led to a period where the duo didn’t have a relationship at all. ![]() Netflix’s ‘ My Unorthodox Life ‘ is a reality series about Julia Haart and her loved ones. Are Robert Brotherton and Ra’ed Saade From My Unorthodox Life Still Together Kumari Shreya. ET spoke with Haart back in April while she promoted her tell-all, Brazen: My Unorthodox Journey from Long Sleeves to Lingerie, where she discussed stepping away from Orthodox Judaism and her pending divorce from Scaglia. Are Robert Brotherton and Ra’ed Saade Still Together My Unorthodox Life Update. ![]() ![]() However, the precise molecular mechanism of FRA2 involved into myocardial I/R injury remains not well explored. Owing to these well-known transcriptional targets of FRA2 are proved to be up-regulated in the tissue fibrosis, FRA2 might function as a crucial driver of myocardial fibrosis. In addition, the level of FRA2 is up-regulation in the infarcted myocardial tissue and implicates in accelerating TGF-β transcription. Previous study indicates that FRA-2 mediates oxygen-sensitive induction of transforming growth factor-β (TGF-β) in cardiac fibroblasts. Consistent with FRA1, the FRA2 lacks C-terminal trans-activating domain. The phosphorylation of FRA2 increases itself DNA binding activity. Once formed, these complexes bind to activator protein 1 (AP-1) sites. FRA2 forms stabilized heterodimer complexes with Jun family members, including c-Jun, Jun-B and Jun-D. However, the roles miR-155 in H/R-induced cardiomyocytes apoptosis and the cardiac fibrosis have not been entirely evaluated.įos-related antigen 2 (FRA2) is a member of Fos family, which contains various immediate-early serum-inducible genes. Altogether, these results imply that therapeutic targeting miR-155 may benefit myocarditis patients. Pharmacological inhibition of miR-155-5p by anti-miRs (antisense oligonucleotides) successfully inhibits cardiac infiltration by monocyte/macrophages, improves cardiac function and attenuates myocardial damage during viral myocarditis (VM). In addition, miR-155 regulates cardiac fibrosis through TGF-β1-Smad2 signaling pathway, which suggests miR-155 may be a potential therapeutic target for preventing cardiac fibrosis. Overexpression of miR-155 in endothelial cell inhibits cells proliferation and re-endothelialization and thus increases the permeability of vascular endothelial. Previously research has suggested miR-155 to be closely associated with cardiovascular heart diseases. Substantial studies have demonstrated that miRNAs play core roles in the regulation of a variety of cellular processes, including cells growth, cellular apoptosis and fibrosis. MicroRNAs is a class of non-coding RNAs, which directly bind to the 3′-UTR region of target genes and induce the degradation of target genes or inhibit the translation of target protein. Thus, reveal the basic molecular mechanisms are urgent for the development effective strategies of myocardial ischemia/reperfusion injury. Suppression of myocardial cell apoptosis and myocardial fibrosis could effectively reduce the degree of myocardial injury caused by I/R as well as improve the survival in patients with heart failure. Ischemia/reperfusion (I/R) injury is one of the mainly causes in cardiomyocyte apoptosis and cardiac fibrosis. Altogether, our results demonstrated that miR-155-5p restrain H/R-induced both cellular apoptosis and fibrosis of cardiomyocytes, partly via directly inhibit FRA2. Moreover, knocked-down of FRA2 accelerated cell growth whereas suppressed the apoptosis and fibrosis in M6200 cells induced by H/R. Both the bioinformatics analysis and luciferase reporter assay demonstrated fos-related antigen 2 (FRA2) is one of the direct targets of miR-155-5p, and miR-155-5p negatively regulated the expression of FRA2 in M6200 cells. Over-expression of miR-155-5p inhibited H/R-induced apoptosis and the fibrosis of cardiomyocyte M6200 cells. Our results implied that H/R induced M6200 cells apoptosis and increased the expression of fibrosis-associated proteins, including collagen I, collagen II, collagen III and fibronectin, as well as decreased the level of miR-155-5p. In this study, cardiomyocyte (M6200 cells) were exposed to hypoxia/reoxygenation (H/R). Nevertheless, the behind mechanisms remain not well investigation. Substantive researches have demonstrated that microRNAs are involved in myocardial I/R injury. Myocardial ischemia/reperfusion (I/R) injury results in cardiomyocytes apoptosis and cardiac fibrosis, which accompanied with fibroblasts trans-differentiate to myofibroblasts and the deposition of collagen. Received: SeptemAccepted: NovemPublished: January 30, 2018 Keywords: hypoxia/reoxygenation cardiomyocyte apoptosis microRNA-155-5p FRA2 Ge Jin 1, Xue Qiang Guan 1, Jia Li 1 and Jun Ma 1ġThe Department of Cardiology, 2nd Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China ![]() |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |